Saline:Article Title: Investigation of miR-652 in Host Immunity Against Intracellular Bacterial Pathogens
Article Snippet: .. Materials 3.1.1 General solutions e Phosphate buffered saline (PBS): Prepared in deionised water from 10X DPBS solution (Gibco). e PBS/Heparin: PBS with 20 U/ml Heparin (Sigma). e FACS wash: PBS supplemented with 2% foetal bovine serum (FBS) (Hyclone), and 2 mM EDTA (Sigma). e ACK lysis buffer: 15 nM NHaCl (Biochemicals), 10 mM KHCO3 (Sigma), and 127 LLM EDTA dissolved in deionised water, adjusted to pH 7.4. e 0.1% Triton solution: 0.1% v/v TritonTM X-100 (Sigma) dissolved in water. e Resazurin solution: 2 mM resazurin sodium salt (Sigma), dissolved in PBS and sterilised by 0.2 um filtration. e Polyethenimine (PEI) solution: 1 g/L polyethylenimine (linear, average Mn 20000) hydrochloride salt (Sigma) dissolved in deionised water, adjusted to pH 7.4. .. 3.1.2 Bacterial culture media e Oleic acid albumin dextrose catalase (OADC) enrichment: 50 g/L bovine serum albumin (BSA) (Moregate Biotech), 20 g/L dextrose (Sigma), 40 mg/L catalase (Sigma), 150 mM NaCl (Sigma), and 1.6 mM oleic acid (Sigma) in water.
FACS:Article Title: Investigation of miR-652 in Host Immunity Against Intracellular Bacterial Pathogens
Article Snippet: .. Materials 3.1.1 General solutions e Phosphate buffered saline (PBS): Prepared in deionised water from 10X DPBS solution (Gibco). e PBS/Heparin: PBS with 20 U/ml Heparin (Sigma). e FACS wash: PBS supplemented with 2% foetal bovine serum (FBS) (Hyclone), and 2 mM EDTA (Sigma). e ACK lysis buffer: 15 nM NHaCl (Biochemicals), 10 mM KHCO3 (Sigma), and 127 LLM EDTA dissolved in deionised water, adjusted to pH 7.4. e 0.1% Triton solution: 0.1% v/v TritonTM X-100 (Sigma) dissolved in water. e Resazurin solution: 2 mM resazurin sodium salt (Sigma), dissolved in PBS and sterilised by 0.2 um filtration. e Polyethenimine (PEI) solution: 1 g/L polyethylenimine (linear, average Mn 20000) hydrochloride salt (Sigma) dissolved in deionised water, adjusted to pH 7.4. .. 3.1.2 Bacterial culture media e Oleic acid albumin dextrose catalase (OADC) enrichment: 50 g/L bovine serum albumin (BSA) (Moregate Biotech), 20 g/L dextrose (Sigma), 40 mg/L catalase (Sigma), 150 mM NaCl (Sigma), and 1.6 mM oleic acid (Sigma) in water.
Lysis:Article Title: Investigation of miR-652 in Host Immunity Against Intracellular Bacterial Pathogens
Article Snippet: .. Materials 3.1.1 General solutions e Phosphate buffered saline (PBS): Prepared in deionised water from 10X DPBS solution (Gibco). e PBS/Heparin: PBS with 20 U/ml Heparin (Sigma). e FACS wash: PBS supplemented with 2% foetal bovine serum (FBS) (Hyclone), and 2 mM EDTA (Sigma). e ACK lysis buffer: 15 nM NHaCl (Biochemicals), 10 mM KHCO3 (Sigma), and 127 LLM EDTA dissolved in deionised water, adjusted to pH 7.4. e 0.1% Triton solution: 0.1% v/v TritonTM X-100 (Sigma) dissolved in water. e Resazurin solution: 2 mM resazurin sodium salt (Sigma), dissolved in PBS and sterilised by 0.2 um filtration. e Polyethenimine (PEI) solution: 1 g/L polyethylenimine (linear, average Mn 20000) hydrochloride salt (Sigma) dissolved in deionised water, adjusted to pH 7.4. .. 3.1.2 Bacterial culture media e Oleic acid albumin dextrose catalase (OADC) enrichment: 50 g/L bovine serum albumin (BSA) (Moregate Biotech), 20 g/L dextrose (Sigma), 40 mg/L catalase (Sigma), 150 mM NaCl (Sigma), and 1.6 mM oleic acid (Sigma) in water.
Filtration:Article Title: Investigation of miR-652 in Host Immunity Against Intracellular Bacterial Pathogens
Article Snippet: .. Materials 3.1.1 General solutions e Phosphate buffered saline (PBS): Prepared in deionised water from 10X DPBS solution (Gibco). e PBS/Heparin: PBS with 20 U/ml Heparin (Sigma). e FACS wash: PBS supplemented with 2% foetal bovine serum (FBS) (Hyclone), and 2 mM EDTA (Sigma). e ACK lysis buffer: 15 nM NHaCl (Biochemicals), 10 mM KHCO3 (Sigma), and 127 LLM EDTA dissolved in deionised water, adjusted to pH 7.4. e 0.1% Triton solution: 0.1% v/v TritonTM X-100 (Sigma) dissolved in water. e Resazurin solution: 2 mM resazurin sodium salt (Sigma), dissolved in PBS and sterilised by 0.2 um filtration. e Polyethenimine (PEI) solution: 1 g/L polyethylenimine (linear, average Mn 20000) hydrochloride salt (Sigma) dissolved in deionised water, adjusted to pH 7.4. .. 3.1.2 Bacterial culture media e Oleic acid albumin dextrose catalase (OADC) enrichment: 50 g/L bovine serum albumin (BSA) (Moregate Biotech), 20 g/L dextrose (Sigma), 40 mg/L catalase (Sigma), 150 mM NaCl (Sigma), and 1.6 mM oleic acid (Sigma) in water.
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